FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24231518
Sequences flagged as poor quality0
Sequence length35-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG80950.016.756951
GTTCTAG79000.016.0721171
GTATTAG56750.015.5326951
GTACTAA56100.015.3055991
CTATACT63200.015.1575964
GTCTTAG65850.015.050791
GTACTAG35300.015.0085481
GTATTAA82450.014.9564471
GTGCTAG78350.014.806421
GGTCTAA52650.014.3133381
GTTCTAA82850.014.1675891
TATACTG74500.014.0831455
GTATATA93850.013.8696641
GTGTTAG78750.013.8612591
GTGCTAA68150.013.13546851
TCTATAC60400.012.9903133
GTATAGG45750.012.8781641
GTCCTAA65350.012.7897151
GTATAAG53950.012.6139241
TCTAGAC67250.012.5489553
Produced by FastQC (version 0.11.5)