FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22174918
Sequences flagged as poor quality0
Sequence length35-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG31250.018.859841
GTATTAG47100.017.460231
GTCCTAG76350.017.353511
GTACTAA49650.017.2996351
TACGCGA5650.016.9697022
GTATTAA73950.016.2485981
GTGCTAG72550.016.0583571
GTCTTAG64100.015.3955091
GGTCTAG57250.014.8434811
GTTCTAA69050.014.8211771
GTTCTAG76550.014.6821011
CTATACT56250.014.283154
GTCCTAA62100.014.2727781
TATACTG72950.014.0795625
GTATAGG43950.014.0337331
GTCTTAA69550.013.9263421
GTACTAT51650.013.6222461
GTGTTAG69100.013.2897371
GTATAAG50200.013.1056061
TTATACT79900.013.0834584
Produced by FastQC (version 0.11.5)