FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28280572
Sequences flagged as poor quality0
Sequence length35-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAA91200.018.3294321
GTACTAG37600.016.7330531
GTATTAG63400.016.0807461
GTCCTAG96150.015.809611
GTACTAA61550.015.4448861
GTTCTAG97700.014.94772151
GTATATA118550.014.4881351
GTCTTAG72000.014.4789081
GCCCTAG101250.014.4689861
GTCCTAT71050.014.1554121
GTTCTAA87650.014.1466711
GTCCTAA76750.013.8221641
GTACTAT59650.013.78113651
TACGCGA11800.013.6069192
GTATTAT90900.012.832531
TCTAGAC75100.012.5214953
GTGTTAG90000.012.4505841
TATACTG91900.012.3288835
GTGCTAG86500.012.3172661
GTCTATA63600.012.2753611
Produced by FastQC (version 0.11.5)