FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28525935
Sequences flagged as poor quality0
Sequence length35-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG42800.018.5284141
GTATTAG65450.018.244991
GTATTAA90750.016.5624491
GTCCTAG96100.016.2161141
GTTCTAG98300.015.9469971
GTCTTAG78100.015.6440211
GTGCTAG92450.015.609571
GTACTAA62700.015.3685281
GTTCTAA95700.014.7422251
GTCTTAA90600.014.5034131
GTATATA113350.014.2770721
GTGCTAA84100.014.1441611
GTATATG97250.013.8909371
GTCCTAA79650.013.5451441
GTCCTAT77350.013.5306621
GTCTATA66600.013.4993831
TACGCGA8200.013.4848312
GTACTAT67950.013.434741
GCTCTAG114500.013.3686061
TATACTG93950.013.3372715
Produced by FastQC (version 0.11.5)