FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28525935
Sequences flagged as poor quality0
Sequence length35-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG66150.018.5456941
GTCCTAG94500.018.057651
GTACTAG42800.017.0256861
GTATTAA99900.016.7583961
GTACTAA63650.016.738051
GTCTTAG81000.015.5442211
GTGCTAG96700.015.5005881
GTTCTAG98850.015.2101061
GTTCTAA96900.014.8974481
TACGCGA10050.014.667952
GTCCTAT76400.014.0050911
GTCCTAA77550.013.9758221
GTCTTAA92000.013.7859341
GCTCTAG109400.013.7011611
GTGCTAA86250.013.421651
GGTCTAA67300.013.2946831
GGTCTAG75450.013.2034021
TATACTG93900.013.1958215
GTATATG96500.013.1430661
GTATATA112450.013.0424441
Produced by FastQC (version 0.11.5)