FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25617645
Sequences flagged as poor quality0
Sequence length35-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG32700.020.3859791
GTCCTAG86850.017.78611
GTATTAG55700.017.4156061
GTTCTAA83250.016.7880671
GTGCTAG82250.016.2655371
GTACTAA54100.016.14611
GTTCTAG86400.015.9099491
GTATTAA77350.015.8694651
GTCTTAA73000.015.4925731
GTATATA86400.015.3246341
CTATACT62800.014.7760064
GGTCTAA55850.014.5700641
GTCCTAA64950.014.2982721
GCTCTAG105900.014.2393191
GTGCTAA69600.014.2017071
GTCTTAG67200.014.1616031
TACGCGA10100.014.1011322
GTACTAT54600.014.0616141
GTATAGG50250.013.9980221
GTACATA72400.013.9064691
Produced by FastQC (version 0.11.5)