FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529590
Sequences flagged as poor quality0
Sequence length100-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACAT7058.398409E-611.488111
CCCAGAG5900.00299545059.9835041
TTCGATC6050.0043166219.5519752
CTCCAAG10456.73652E-69.1595081
CTCATGT6450.0062499639.1321971
CAATCAC10150.00398151257.2540241
CTCAAGA10400.0050455337.07964851
CTTCGAT11550.00179397727.01222321
GTCAAGA18905.2925334E-66.62265541
GGATTGT12600.00447400246.4278711
CTCAAAA20401.7281409E-56.13569551
CTTGAAT14450.00274505126.11446481
CTCAAAC13400.0084807156.0441181
CTCTTCT16050.00135467425.96366641
CTCCGTT14850.00372428945.9497651
CTTTTAT29803.2750904E-85.92979961
CCTTCAT21403.600028E-55.84898041
CTTCAAT22701.416823E-55.8383711
CAGATTT18803.0235108E-45.7631993
CTCAACG15800.0074080975.5920261
Produced by FastQC (version 0.11.5)