FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2323576
Sequences flagged as poor quality0
Sequence length100-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC1100.00987747826.2513738
CTTAGTA5850.00318423439.9105191
CCCACGA8750.00108255098.2823631
CCCAAAG13553.0170831E-68.0225841
CCCAGTT13853.477619E-57.32555531
CACCAAT18255.168804E-77.14779231
CTCACTT11600.00222069656.87221861
CCCCATT14153.5785703E-46.6580831
CGAACAT14304.069236E-46.58824251
CTTAATC14404.4293777E-46.5424911
CTCAAAG24804.6667992E-86.42884971
CCTCGAA12550.00506650336.35201071
CTCCGAA16302.9235633E-46.22447441
CTCAAGG18653.8901424E-56.2173231
CCCATCT16453.290338E-46.1677171
CTCAAAT23709.121013E-76.1156681
CTTGAAT21457.3406645E-66.0814551
CCCTCTT16703.9956742E-46.07538561
CACAAGT13300.0092507215.9938151
CTTTAAA34005.875336E-105.9681731