FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1733382
Sequences flagged as poor quality0
Sequence length100-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGT1050.00793736927.4550152
CTTTAGA5850.002845361110.0457691
CTCAAGG16154.60308E-77.7325981
CTCGAAT8800.0092863887.5129221
CCCCCCC5952.4820127E-57.509278145
CTCAGAA9950.00335341767.3828831
CCCTCAT12857.4851466E-46.8600491
CTTCAAT23005.8853402E-86.7071881
CCACTGT11050.0092772396.64793541
CTTTAAG12650.0047765336.38779831
AATTAAT14100.0021397986.2518881
CTCGAAG14550.00304300666.05853031
CTTTCAA25855.31998E-75.96771141
CTTCAAG24751.5509722E-65.93613621
CCCTTCT14950.0041181545.8964291
CCTCAAA16300.00167575375.85874751
ATGATCA16450.00234741165.6954553
GTCCTTT19554.0892407E-45.6362931
ATTCGGA15650.00684015135.6326911
CTTTAAT17100.00296396445.58465431
Produced by FastQC (version 0.11.5)