FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005000143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110443942
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG475500.016.0693599
GAGCGTC579050.013.3581598
AGAGCGT666300.011.6955337
ATCGGAA737350.010.7704851
CGCCGTA179700.010.5698545-49
TCGGAAG753800.010.532752
AAGAGCG799950.010.0495576
CGGAAGA799400.09.9673073
GTCGCCG235500.08.62031240-44
GCCGTAT238250.08.36787945-49
TGGTCGC272200.07.569928640-44
GCGTCGG79650.07.557646145
TCGCCGT205500.07.45845740-44
GGTCGCC280700.07.39752540-44
CCGTATC273550.07.335757745-49
CGTATCA282500.07.098217545-49
GTGGTCG298500.06.873816540-44
GAAGAGC1543750.05.6301895
TCGGTGG393100.05.54097135-39
TCTCGGT400300.05.448553635-39
Produced by FastQC (version 0.11.5)