FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005000392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79835797
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG145050.012.5525645-49
ATCGGAA578850.011.6965021
TCGGAAG587050.011.5144262
CGGAAGA609450.011.0717073
CTCGTAT169000.011.00614640-44
CGTATGC175150.010.6196940-44
TCTCGTA172100.09.91495940-44
ATGCCGT199450.09.41230945-49
TCGTATG203250.09.372601540-44
GCCGTCT201350.09.35948945-49
TGCCGTC202800.09.32116145-49
CGTCTTC253350.07.512696350-54
AGAGCAC1025000.07.04263977
AGCACAC1044450.06.8698639
GAGCACA1093050.06.65060958
GAAGAGC1145500.06.33343035
GATCGGA521100.06.09306145
ATCTCGT214650.06.06488935-39
AGATCGG546400.05.930335145
AATCTCG330150.05.761027335-39
Produced by FastQC (version 0.11.5)