FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15155122
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG21400.07.717583745-49
CGTACGA19450.07.57687230-34
GATCGGA94100.07.29144861
TCGGAAG98650.07.19599253
CCGTACG20250.07.061374730-34
ATCGGAA98600.06.98472832
CTCGTAT26100.06.54985640-44
CGGAAGA104400.06.45273974
TCTCGTA26350.06.26779140-44
TACGATC23850.06.168003635-39
ACACGTC69200.05.8628416145
CGTATGC27600.05.826452340-44
AGATCGG97900.05.624152145
ATGCCGT31450.05.43565245-49
ACCGTAC27600.05.39236530-34
TCGTATG31150.05.301967640-44
TGCCGTC31800.05.284709545-49
GCCGTCT33200.05.01822445-49