FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15155122
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG12307.508788E-89.425554145
GAGCGTC79600.08.64825259
CGCCGTA18500.08.46051345-49
AGAGCGT90550.07.68243938
AGCGTCG64900.07.2570596145
GATCGGA97200.06.9869491
ATCGGAA101050.06.74067832
TCGGAAG103400.06.7276633
AAGAGCG109650.06.67461547
GTCGCCG26200.06.58263540-44
CGGAAGA111450.06.43677954
TCGCCGT22650.06.334489345-49
GCCGTAT26600.06.265573545-49
GTCGTGT63250.05.957099145
CGTCGTG57350.05.685532145
AGATCGG101050.05.521364145
GCGTCGT58350.05.364736140-144
CCGTATC30550.05.360581445-49
TGGTCGC33200.05.325689340-44
GTGGTCG33750.05.281841840-44
Produced by FastQC (version 0.11.5)