FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005000516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110474716
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC8955950.8106787076963384TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2175100.072.863061
ATCGGAA2194300.072.326382
TCGGAAG2191000.071.689493
CGGAAGA2216650.070.324434
AGAGCAC2752600.056.281858
GAGCACA2855150.054.2628759
GAAGAGC2927200.053.184826
AAGAGCA3468350.044.940997
GGAAGAG3847650.040.815865
GATCGGG144650.034.2592121
ATCGGGA149250.032.8031042
TATGCCG1344400.024.49409145-49
CTCGTAT1370650.023.83944940-44
TCTCGTA1281450.023.50748840-44
ATGCCGT1398700.023.46129245-49
TCGTATG1424150.023.1424240-44
TGCCGTC1422800.023.0139645-49
CGATCAG1451900.022.62282230-34
CGTATGC1392250.022.31777640-44
CGTCTTC1486450.021.9394850-54
Produced by FastQC (version 0.11.5)