FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005000519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108065115
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG9609610.8892425645408326Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC1881550.079.61229
AGAGCGT1965000.076.3584068
GATCGGA2089550.071.6477661
ATCGGAA2123050.071.266572
TCGGAAG2134800.070.760683
AAGAGCG2141000.070.611757
CGGAAGA2171700.069.5903554
GAAGAGC2868500.052.8827366
GGAAGAG3768600.040.4472055
CGCCGTA1214450.025.14875245-49
GCCGTAT1256300.024.34562345-49
TCGCCGT1245300.023.95398945-49
CCGTATC1291500.023.73821645-49
GTCGCCG1293050.023.67799240-44
CGTATCA1298650.023.49474345-49
TGGTCGC1322150.023.2369140-44
GGTCGCC1340650.023.00602540-44
GTGGTCG1350750.022.7620840-44
TCGGTGG1473050.021.0485735-39
TCTCGGT1473750.021.00905635-39
Produced by FastQC (version 0.11.5)