FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34823578
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG636930.18290194074830565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT20850.013.3340251
GTCTAAC49400.011.8844194
TAACACG52250.011.6366677
CGACCTA23350.011.4557114
TGCGCGA53400.010.93382614
TCGCACG54400.010.72875230
CGCGAGT58900.010.26688616
CGCACGA56450.010.15377731
ACGTGCG60100.010.11923811
CGAGTCG61350.09.63024318
TACGCAC32950.09.1753339
GCGCGCT96400.09.09727470
GCGATAG28000.08.97313966
CGCGCTA29500.08.83209546
GTAGGTC41700.08.8305942
TCCGCAT79900.08.79397270
GCGCTAA42150.08.73601642
ACCGTTG29450.08.728393
AACACGT69950.08.3948338
CCGTTGA33250.08.2537954
Produced by FastQC (version 0.11.5)