FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23810098
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG242880.10200713999581187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGATT41450.018.78242149
TTCTCGA41350.018.65969847
TCTCGAT42000.018.37095848
ATTCCGT40500.018.11418753
TCGATTC44700.017.02843550
CGACCGT21150.016.9291531
GTCTAAC45100.016.7219414
GTACGCA34200.016.6669450
TCCGCAT76800.016.57768470
ACGGACA49700.016.29632618
ACGCACG38550.015.33051352
ATCGACC50600.015.314445543
CGACCTA16650.014.8199794
ATAGCTC54950.014.73297539
GTAGGTC47300.014.6937652
CCGGTAC39800.014.59594260
CGCAAAA68800.014.0463911
TACGCAC41050.013.97178351
TGCGCGA52850.013.68128114
GACCGTT25450.013.6545222
Produced by FastQC (version 0.11.5)