FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44339249
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN588740.13278077849266234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA56300.022.4049514
CGTATCG66550.022.2959543
CCCGTAT71250.021.1230051
CCGTATC82700.019.8332792
TATCGAA67650.019.7760315
TACCGGC45550.015.28158170
ACTATCG70600.014.00238458
ATCGAAG125350.013.7777486
TACATCG93050.013.49449564
CGTGTTA94250.013.26206159
CTATCGG77050.013.01420859
ATCGCGC97000.012.66275467
GTATACG58900.012.21221312
TATACGG60000.011.698750513
TATCGGT87100.011.63540260
ACATCGC113700.011.41758165
CGCGCCA119850.011.39002569
TTACGGT90800.011.21661843
GCCGTAT48350.010.8550821
CGCGTTA36300.010.72204834
Produced by FastQC (version 0.11.5)