FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005000696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56549459
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGTTACATGTGTAGATCT649570.11486758874209567Illumina Single End PCR Primer 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC486100.022.1799899
AGAGCGT522250.020.81248
GATCGGA552750.019.7714061
ATCGGAA577700.019.0656382
TCGGAAG599000.018.411843
AAGAGCG607200.018.091597
CGGAAGA620000.017.8232754
CGCCGTA161150.017.04364855-59
TCGCCGT179800.015.09842955-59
GCCGTAT186200.014.70401655-59
GTCGCCG206150.013.21073955-59
CGTATCA209550.013.13978460-64
TGGTCGC214050.012.89333450-54
GTGGTCG223450.012.39635150-54
GAAGAGC986400.011.6141696
CCGTATC204300.010.50531360-64
TCTCGGT280600.010.22179945-49
GGTCGCC231150.09.34277855-59
CGGTGGT301000.09.34219250-54
CTCGGTG312550.09.19080145-49
Produced by FastQC (version 0.11.5)