FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55641591
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTTCATAGATCTCGTAT881170.15836534940203276TruSeq Adapter, Index 27 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA308800.050.3128431
ATCGGAA330400.047.232332
TCGGAAG344900.045.0173153
CGGAAGA372200.041.7348754
AGAGCAC689750.022.7095958
GAGCACA744950.021.182529
GAAGAGC762050.020.659676
TATGCCG196750.017.97837845-49
CTCGTAT212800.017.34473840-44
TCTCGTA204900.016.44277640-44
ATGCCGT231100.015.88951745-49
CGTATGC214400.015.17983345-49
AAGAGCA1063150.015.0607817
GCCGTCT231200.013.9200650-54
TCGTATG227550.013.76744545-49
ATCTCGT234000.013.63594240-44
TGCCGTC230950.013.52084150-54
GGAAGAG1214550.013.4044095
CGTCTTC268500.013.26590350-54
CCGTCTT272850.012.32119350-54
Produced by FastQC (version 0.11.5)