FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005000700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57895257
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCTGAAACGTGTAGATCT1675470.2893967635379872Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC457050.055.5626539
GATCGGA500250.051.1659051
AGAGCGT498600.050.9627048
ATCGGAA522200.049.059092
TCGGAAG534900.047.870523
AAGAGCG557450.045.7126167
CGGAAGA565000.045.4356584
GAAGAGC947400.027.5324256
CGCCGTA227800.022.79946355-59
TCGCCGT242350.021.41868255-59
GTCGCCG259100.020.19072355-59
GCCGTAT259700.019.99891555-59
GGAAGAG1406300.018.898785
TGGTCGC278850.018.72412550-54
CGTATCA285450.018.30644260-64
CCGTATC277750.018.28669760-64
GTGGTCG290000.018.1292150-54
GGTCGCC283550.018.0968855-59
TCTCGGT340450.015.63015345-49
TCGGTGG337950.015.21371850-54
Produced by FastQC (version 0.11.5)