FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005000741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60637451
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAGCTGATCTCGTAT1162440.19170330890063303TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA719700.027.2543351
ATCGGAA746650.026.4860082
TCGGAAG750150.026.180093
CGGAAGA766300.025.7229634
TCCGATC56100.019.2545623
AGAGCAC1047450.019.0608528
GAGCACA1114500.017.99879
TATGCCG270350.017.83241745-49
GAAGAGC1151600.017.4880836
CTCGTAT288000.016.45261640-44
CGTATGC292800.016.24230445-49
TCTCGTA276600.016.00892840-44
ATGCCGT305750.015.72034145-49
GCCGTCT314600.014.94702350-54
AAGAGCA1431800.014.2834267
CCGATCT78450.014.1386884
ATCTCGT313550.014.02526240-44
CGTCTTC349850.013.80987350-54
TTCCGAT81950.013.5490712
CCGTCTT356850.013.32768350-54
Produced by FastQC (version 0.11.5)