FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005000742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60637451
Sequences flagged as poor quality0
Sequence length151
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTACAGGTCGTGTAGATCT1059010.17464619348857524Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC619700.029.0045979
GATCGGA702200.027.1640151
AGAGCGT669650.027.0019158
ATCGGAA730100.026.0036412
TCGGAAG736100.025.5863423
CGGAAGA761650.024.5869984
AAGAGCG753000.024.279767
CGCCGTA238700.019.27291555-59
TCGCCGT260150.017.5792155-59
GCCGTAT263000.017.49763155-59
GAAGAGC1129150.016.5658196
GTCGCCG284850.015.79341455-59
CGTATCA290150.015.41045160-64
TGGTCGC306350.015.18083550-54
GTGGTCG315900.014.74910550-54
TCTCGGT361550.013.05228545-49
AGGTCGT343500.012.98760635-39
GGTCGTG369350.012.48189835-39
GGAAGAG1568100.012.4354775
CTCGGTG394250.012.03556845-49
Produced by FastQC (version 0.11.5)