FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55366749
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAGCTGATCTCGTAT1224530.2211670401670143TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA860450.024.167671
TCGGAAG881500.023.6529713
ATCGGAA889000.023.5377432
CGGAAGA888100.023.4935174
TCCGATC50500.021.3901583
AGAGCAC1119650.018.7838338
GAGCACA1157200.018.212619
TATGCCG297350.017.67635245-49
GAAGAGC1220900.017.3686036
CGTATGC323300.016.03778545-49
CTCGTAT323250.015.97293640-44
ATGCCGT326400.015.88102345-49
TCTCGTA311950.015.64982340-44
GCCGTCT333400.015.33441450-54
CCGATCT72700.014.5592014
AAGAGCA1471500.014.5141367
TTCCGAT76750.014.1753452
CGTCTTC368550.014.02534650-54
ATCTCGT344450.013.98799540-44
CCGTCTT374750.013.75074650-54
Produced by FastQC (version 0.11.5)