FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005000744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55366749
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTACAGGTCGTGTAGATCT1114960.20137718398456086Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC712450.026.5490869
AGAGCGT772750.024.6181098
GATCGGA859250.023.8610571
ATCGGAA882700.022.9568082
TCGGAAG879200.022.7674563
CGGAAGA897650.022.1622184
AAGAGCG880150.022.0424217
CGCCGTA258050.019.47495355-59
GCCGTAT279150.018.00290755-59
TCGCCGT278550.017.83867655-59
GAAGAGC1210000.016.3631086
GTCGCCG301550.016.26170255-59
CGTATCA306800.015.978785560-64
TGGTCGC318950.015.78369850-54
GTGGTCG329850.015.37201450-54
TCTCGGT379200.013.64678945-49
AGGTCGT379600.012.82660235-39
CTCGGTG407300.012.76936245-49
GGTCGTG398300.012.69036635-39
GCGTCGG71400.012.590572145
Produced by FastQC (version 0.11.5)