FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005000793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75314315
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGCATAATCTCGTAT870550.11558891559990951TruSeq Adapter, Index 5 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA704650.020.8495581
ATCGGAA716600.020.6075732
TCGGAAG731700.020.0611023
CGGAAGA748450.019.5854954
TATGCCG200150.015.95917145-49
CTCGTAT231700.013.47305540-44
CGTATGC230350.013.30663345-49
TCTCGTA230100.013.30208340-44
AGAGCAC1136250.013.0923338
ATGCCGT243150.012.89237245-49
GAGCACA1205650.012.3267729
GAAGAGC1252000.012.0209626
TCGTATG247650.011.96723945-49
GCCGTCT261900.011.73665550-54
TGCCGTC258000.011.47010250-54
ATCTCGT277400.010.74121640-44
CGTCTTC308600.010.61363950-54
CCGTCTT313150.09.98715350-54
AAGAGCA1630950.09.5124027
GGAAGAG1897600.08.3438045
Produced by FastQC (version 0.11.5)