FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005000801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62409889
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA279250.023.743591
ATCGGAA328600.020.2604942
TCGGAAG337800.019.6372093
CGGAAGA360550.018.4182474
TATGCCG140950.016.41830645-49
CTCGTAT163200.014.0555340-44
CGTATGC160150.013.96104545-49
TCTCGTA169750.013.248383540-44
ATGCCGT179750.012.82592745-49
GCCGTCT190300.011.7643650-54
ATCTCGT200550.011.07635940-44
CGTCTTC232700.010.13173950-54
CCGTCTT232050.09.92267550-54
AGAGCAC707500.09.7051138
CATCTCG231350.09.63308240-44
GAGCACA759650.09.2393199
GAAGAGC799700.08.9566376
CGTCTGA255250.08.86723115-19
ACACGTC264850.08.65653310-14
TCGTATG176400.07.956779545-49
Produced by FastQC (version 0.11.5)