FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005000816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63499544
Sequences flagged as poor quality0
Sequence length151
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG70100.011.478349145
CGCCGTA108550.08.134418555-59
TCGCCGT127900.06.971779355-59
GCCGTAT134650.06.89148355-59
GAGCGTC579450.06.0923862145
CGTCGTG443150.05.888792145
AGCGTCG493350.05.65692145
AGATCGG798350.05.647699145
CGTGTAG447050.05.6266227145
TCGGAAG768550.05.583725145
CGTATCA161950.05.5692860-64
TCGTGTA436500.05.5467257145
GTCGCCG160250.05.50103755-59
ATCGGAA769050.05.4481335145
GCGTCGT450350.05.4308615140-144
TGGTCGC171950.05.429977450-54
GATCGGA762250.05.4016366145
AAGAGCG755700.05.1702766145
GTCGTGT451000.05.1433716145
GTGGTCG183300.05.10166150-54
Produced by FastQC (version 0.11.5)