FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56488716
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGGTGATCTCGTAT647960.11470609457648144TruSeq Adapter, Index 3 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTG83050.041.744962
CACCGGT86200.040.6056441
CCGGTGG148400.023.5430133
CGGTGGT168900.020.9859264
GATCGGA701100.016.2751791
TCGGAAG720050.015.9858873
ATCGGAA715100.015.9847272
CGGAAGA732150.015.6820934
TATGCCG194350.014.64939545-49
ACGACGC47250.014.23604125-29
CGACGCT50850.013.71283225-29
CGTATGC217800.012.98560445-49
TCTCGTA215250.012.79611740-44
CTCGTAT218900.012.67546640-44
CACGACG54150.012.28817225-29
ATGCCGT229950.012.17969645-49
AGAGCAC953450.012.0721698
GTAACAC303700.011.9570949
GAGCACA990800.011.5585639
GCCGTCT244800.011.52981250-54
Produced by FastQC (version 0.11.5)