FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32043833
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC2965090.9253231347198696TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA507350.0103.231261
ATCGGAA520550.0100.8591842
TCGGAAG521300.099.338343
CGGAAGA535050.096.013274
AGAGCAC715350.071.215248
GAGCACA747850.068.052539
GAAGAGC767900.066.568296
AAGAGCA930200.055.0782367
GGAAGAG1027850.050.275765
GATCGGG41750.048.9440461
ATCGGGA44750.044.065152
TCGGGAA64200.030.711223
TATGCCG419000.026.02757645-49
CTCGTAT424900.025.46922740-44
TCTCGTA402450.025.27240640-44
CGATCAG433050.025.14389830-34
ATGCCGT432900.025.09808345-49
TCGTATG441200.024.6860140-44
TGCCGTC437650.024.68323545-49
ACGATCA452950.024.2024730-34
Produced by FastQC (version 0.11.5)