FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005000968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68368588
Sequences flagged as poor quality0
Sequence length151
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA168100.016.19075855-59
TCGCCGT189200.014.40812155-59
GCCGTAT196200.013.99014655-59
AGCGTCG579750.013.130529
GCGTCGG69200.012.571375145
TGGTCGC230600.012.02214750-54
GTCGCCG218200.011.622663555-59
GAGCGTC665950.011.4962378
GTGGTCG244600.011.339972550-54
CGTATCA230000.011.03967460-64
AGAGCGT718950.010.7089957
CGGTGTA269600.010.27199835-39
AAGAGCG830050.09.37176
ATCGGAA839850.09.3349431
TCGGAAG845700.09.2669072
CGGAAGA857000.09.1448183
TCTCGGT310850.08.99308945-49
ACCGGTG308900.08.88534335-39
CCGGTGT331750.08.45690635-39
CTCGGTG342950.08.3246845-49
Produced by FastQC (version 0.11.5)