FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42350467
Sequences flagged as poor quality0
Sequence length151
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC366150.013.9004959
CGCCGTA101600.013.8852755-59
AGAGCGT398000.012.8609888
GATCGGA430800.012.0565891
TCGCCGT118250.011.99148455-59
GCCGTAT120900.011.836575555-59
ATCGGAA449200.011.6213812
TCGGAAG458900.011.3750633
AAGAGCG460900.011.2000737
CGGAAGA468050.011.1993454
CGTATCA137750.010.48337560-64
GTCGCCG138550.010.24498655-59
TGGTCGC149500.09.68847450-54
GTGGTCG156200.09.347157550-54
GCGTCGG47750.08.654733145
CCGTATC134600.07.72338160-64
TCTCGGT193550.07.610857545-49
GAAGAGC732800.07.44011166
CGGTGGT213150.06.94498850-54
CTCGGTG218650.06.830001445-49
Produced by FastQC (version 0.11.5)