FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005001000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005001000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54974736
Sequences flagged as poor quality0
Sequence length151
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG60850.016.797897145
CGCCGTA100200.07.59723255-59
CGTCGTG500900.06.411345145
GCCGTAT121850.06.366372655-59
AGCGTCG557250.06.322409145
TCGCCGT123200.06.308380655-59
CGTGTAG484950.06.2036543145
GTCGTGT495200.05.9142156145
GCGTCGT511800.05.8614297140-144
TCGTGTA475650.05.715315145
AGAGCGG159500.05.635824145
GATCGGA843750.05.6018343145
ATCGGAA842000.05.5445995145
GAGCGTC626700.05.4945273145
AGATCGG885650.05.4677763145
GTCGCCG141900.05.35442355-59
CGTATCA147400.05.341579460-64
TAGATCG289650.05.2808685145
AAGAGCG810050.05.208439145
AGAGCGT691250.05.170411140-144
Produced by FastQC (version 0.11.5)