FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005001005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005001005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65354948
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAC48200.014.9089981
GCGACCA72050.012.2768633
CGACCAC84950.010.66875654
GGCGACC83550.010.5920822
ACCGAGA122550.07.86806549
TATGCCG94050.07.584911345-49
CCACCGA131300.07.23294077
CACCGAG157900.06.4278878
ACCACCG158400.06.27008966
AGATCGG362050.06.1273103145
CTCGTAT120700.06.09028940-44
GATCGGA341600.05.942335145
ACGACGC32450.05.808618545-49
TCTCGTA125950.05.767356440-44
CGTATGC118850.05.697205545-49
CGACGCT34400.05.521545-49
ATGCCGT140600.05.321187545-49
ATCGGAA366550.05.28379542
TCGGAAG383850.05.25103333
CGGAAGA406250.05.03295374
Produced by FastQC (version 0.11.5)