FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005001018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005001018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52486873
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTACAGGTCGTGTAGATCT709120.13510425740165546Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC399200.028.3318469
AGAGCGT437300.026.1286858
GATCGGA451900.025.944821
ATCGGAA475800.024.6687952
TCGGAAG500200.023.4075533
CGGAAGA515900.022.5686234
AAGAGCG521100.022.1347927
CGCCGTA157450.018.134555-59
GCCGTAT177650.016.14595255-59
TCGCCGT179600.016.05946455-59
CGTATCA199450.014.27209460-64
GAAGAGC846100.014.0266026
GTCGCCG203950.013.99988955-59
TGGTCGC212950.013.61218250-54
GTGGTCG225750.012.9495750-54
AGGTCGT229400.012.22353935-39
CCGTATC196650.011.37819560-64
TCTCGGT264750.011.23382345-49
GGTCGTG258400.011.19958435-39
CTCGGTG297100.010.15216345-49
Produced by FastQC (version 0.11.5)