FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005001023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005001023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70289368
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGAAATCATCTCGTAT1007520.14333889017183937TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA343650.050.3383671
ATCGGAA381950.045.438892
TCGGAAG396600.043.716593
CGGAAGA423950.040.7766574
AGAGCAC821650.021.410668
GAGCACA884200.019.7894579
TATGCCG209900.019.50442545-49
GAAGAGC914050.019.2697476
TCTCGTA225300.017.4381440-44
CTCGTAT232350.017.22100440-44
CGTATGC234850.017.0002145-49
ATGCCGT257750.015.60792645-49
GCCGTCT263150.015.17807550-54
ATCTCGT264850.014.64252140-44
AAGAGCA1295050.013.9084767
TCGTATG256100.013.79516445-49
TGCCGTC260800.013.35809850-54
CGTCTTC312700.013.3061550-54
CATCTCG295650.013.00922540-44
CCGTCTT313700.012.93098550-54
Produced by FastQC (version 0.11.5)