FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005001038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005001038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59083436
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA57550.011.61471555-59
GAGCGTC221100.010.7884459
GATCGGA245450.09.6125311
AGAGCGT264450.09.0747818
TCGCCGT74100.09.04017355-59
ATCGGAA269900.08.8914612
TCGGAAG283700.08.4590263
GCCGTAT88750.07.79296955-59
CGGAAGA316550.07.695714
GTCGCCG91150.07.33326255-59
AAGAGCG331600.07.3027047
GCGTCGG48600.07.0111766145
TGGTCGC106050.06.576388450-54
AGCGTCG166450.06.1852369
CGTATCA115350.06.159302760-64
GTGGTCG120150.05.852899650-54
Produced by FastQC (version 0.11.5)