FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005001079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005001079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63394755
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA322450.013.5380611
ATCGGAA357500.012.2555292
TATGCCG113650.011.96684445-49
TCGGAAG370550.011.8235973
CGGAAGA392850.011.1104124
CTCGTAT131550.010.39354840-44
CGTATGC133300.010.03963245-49
TCTCGTA129000.09.92462340-44
ATGCCGT157900.08.82444745-49
GCCGTCT164050.08.14015450-54
ATCTCGT164350.07.948735740-44
CGTCTTC207100.06.74910550-54
CCGTCTT208500.06.71074150-54
AGAGCAC743000.06.2836988
AGATCGG323950.06.155365145
TCGTATG152350.06.06238145-49
GAAGAGC833550.05.96637776
GAGCACA797550.05.86300239
TGCCGTC160950.05.77452550-54
CGTCTGA260900.05.323931715-19
Produced by FastQC (version 0.11.5)