FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005001137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005001137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55495479
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGCAAAATCTCGTAT2674680.4819635848174227TruSeq Adapter, Index 25 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA947800.050.7920461
ATCGGAA961800.050.1588862
TCGGAAG963950.049.721693
CGGAAGA970100.049.339494
AGAGCAC1211750.039.4808968
GAGCACA1247950.038.3705069
GAAGAGC1315650.036.6164746
AAGAGCA1552300.031.1510077
GGAAGAG1749200.027.8637565
TATGCCG453250.023.50119645-49
CTCGTAT473150.022.11242340-44
TCTCGTA470200.022.06919940-44
CGTATGC473000.022.03554345-49
ATGCCGT485200.021.54423545-49
GCCGTCT501300.020.65952350-54
ATCTCGT500600.020.41325640-44
CGTCTTC534900.019.84162750-54
TCGTATG488950.019.57888445-49
CCGTCTT535850.019.47359350-54
TGCCGTC496850.019.2072350-54
Produced by FastQC (version 0.11.5)