FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005001198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005001198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65476242
Sequences flagged as poor quality0
Sequence length151
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA174950.018.57336655-59
GAGCGTC547900.016.764759
TCGCCGT196800.016.29756555-59
GCCGTAT203050.016.1101255-59
GATCGGA630250.015.8070851
AGAGCGT606600.015.310168
GTCGCCG216900.014.50650755-59
ATCGGAA689450.014.385052
AGCGTCG475200.014.2797699
TCGGAAG694700.014.1197463
TGGTCGC238900.013.88594950-54
AAGAGCG708500.013.6600387
GTGGTCG246900.013.57695750-54
CGGAAGA729250.013.520384
CGTATCA237750.013.22216160-64
CGGTGTA253200.013.210546535-39
ACCGGTG286500.011.5991735-39
TCTCGGT305300.011.37462545-49
CCGGTGT306200.010.985496535-39
CACCGGT291700.010.89037635-39
Produced by FastQC (version 0.11.5)