FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005001204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005001204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38515523
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTTTTGAGTGTAGATCT409680.10636750278582482Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC283000.022.5182579
AGAGCGT311350.020.5377128
GATCGGA333950.019.2876741
ATCGGAA344950.018.6212222
CGCCGTA83450.018.34897255-59
TCGGAAG355650.018.1426723
AAGAGCG359700.017.9179137
CGGAAGA366100.017.6050454
TCGCCGT98200.015.68148655-59
GCCGTAT101450.015.16482855-59
GTCGCCG112650.013.78581555-59
CGTATCA121100.012.80074660-64
TGGTCGC123100.012.6032750-54
GTGGTCG127400.012.25755250-54
CCGTATC117750.010.83735860-64
GAAGAGC622600.010.7477796
GGTCGCC126050.010.21513855-59
GCGTCGG37050.010.174843145
TCTCGGT159850.09.99599245-49
CGGTGGT172700.09.42015450-54
Produced by FastQC (version 0.11.5)