FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005001273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005001273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53995446
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA421250.020.3554421
ATCGGAA444200.019.4363162
TCGGAAG448450.019.2849583
CGGAAGA460500.018.8433114
TATGCCG134050.016.22389845-49
CTCGTAT149100.014.46005740-44
CGTATGC149650.014.15481745-49
TCTCGTA150850.013.96551540-44
TATCTCG159600.012.78197340-44
ATGCCGT168250.012.74511745-49
AGAGCAC736050.012.1239648
GCCGTCT177900.011.88273250-54
GAGCACA786100.011.398239
ATCTCGT186550.011.27739540-44
GAAGAGC831300.010.8743016
CGTCTTC212450.010.35976250-54
TCGTATG169300.010.276731545-49
CCGTCTT218850.09.96405350-54
TGCCGTC175300.09.80928950-54
AAGAGCA1097100.08.4313747
Produced by FastQC (version 0.11.5)