FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005001332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005001332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51486115
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA88300.012.90040355-59
TCGCCGT100450.011.16689655-59
GCGTCGG44400.010.616509145
GCCGTAT110750.010.48166455-59
GTCGCCG113050.09.84537155-59
TGGTCGC137800.08.63405850-54
GTGGTCG142650.08.44209650-54
CGTATCA139150.08.113358560-64
AGCGTCG341850.07.77894459
GAGCGTC393500.07.42079649
CGGTGTA157300.07.41664535-39
TCTCGGT186400.06.76428345-49
AGAGCGT444000.06.6746758
ACCGGTG178000.06.52972235-39
GATCGGA488450.06.26234631
CTCGGTG209050.06.14231445-49
CCGGTGT194850.06.098932335-39
CACCGGT184950.06.041438635-39
AAGAGCG519600.06.0244987
ATCGGAA512500.06.02378652
Produced by FastQC (version 0.11.5)