FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005001994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005001994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444781
Sequences flagged as poor quality0
Sequence length100-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT2056.1720675E-726.9464197
CACGTCT2301.6830636E-624.0174629
GCACACG3002.8514478E-823.019326
ACACGTC2503.4732511E-622.0960668
GAGCACA2956.1709034E-721.0685314
AGAGCAC2901.2528499E-519.0504723
CTCCCGT2450.001624987917.0019991
AGCACAC4051.2708888E-515.3462125
CTCCGAT4303.461925E-412.9162471
GAAGAGC6054.0522893E-511.4751791
CTAAAAT4900.001038318211.3346661
ATATCGG6200.007635038.9097028
Produced by FastQC (version 0.11.5)