FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005002255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39144227
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA45450.022.7950024
CGTATCG62300.021.2057363
TATCGAA52950.020.879425
TACCGGC39650.018.63533670
CCCGTAT69150.018.4563371
CCGTATC82300.017.4892462
ACTATCG58750.015.75341558
CTATCGG61100.014.97844159
ATCGCGC71900.013.8828467
TACATCG73500.013.45698464
CTACCGG42150.013.41255569
CGTGTTA75200.012.91036359
ATCGAAG115300.012.5134226
TCGACCG17850.012.46557631
ACATCGC86300.012.10977765
GACCGTT46350.012.00144826
TATCGGT80600.011.57192960
ACGGTAC78500.011.51855845
CGACCGT19650.011.50060932
CGCGTTA36950.011.19690834
Produced by FastQC (version 0.11.5)