FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005002256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39144227
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA42550.018.860384
TTAGCGC20850.017.6622312
CTTAGCG37700.014.7507721
GCGAACG34200.014.6270653
TACGCAC61950.013.91204559
TCGTAAG30250.013.2082386
TAGGTCG29100.012.8929283
GTCTAAC55150.012.7245914
TTAGCGG42450.011.9524199
TGCGCGA62650.011.92742914
GTCGTAA34400.011.917345
ACGTGCG61300.011.7360711
TCCGCAT114600.011.64300970
GGTCGTA38500.011.5501394
CGCACGA63350.011.40688231
CGCGAGT61950.011.3890816
GAGTAGG110850.011.22163970
CGACCGT28000.011.1717341
CATATAG93050.011.13056312
CGGCGAA49800.011.0969581
Produced by FastQC (version 0.11.5)