FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005002260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40722581
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG532280.13070880747956523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA37400.016.8135994
CTTAGCG30350.013.28457451
TTAGCGC20150.013.2761952
GCGAACG35550.012.6059943
TACGCAC54750.012.3142619
TTTGCCG113000.012.175162
TAGGTCG28950.011.8799233
GTAGGTC44700.011.813922
ATTGTCG167500.011.27305270
TCGCGAC24850.010.90418234
GTCGACT38100.010.8560141
TTGCCGC121800.010.8383533
CTTGACG49000.010.4981791
GTCTAAC51000.010.286334
CGACCGT24850.010.210451
TAACACG52500.010.1924257
TACCGGT41600.010.10515644
TTGTCGT112200.010.03096470
TGCGCGA55850.09.8327714
ACCGGTA43500.09.74369645
Produced by FastQC (version 0.11.5)