FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005002264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37309178
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG501360.13437980327521556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA39350.021.1841684
GCGAACG33700.017.4172523
TACGCAC56350.015.4150839
CGGCGAA45850.013.490361
GGCGAAC44800.012.7151942
TAACACG54200.011.537017
CACGCTA49000.011.34139637
CTACGCA77600.011.3271078
GTCTAAC52750.011.3253324
TTAGCGC15950.011.3239982
GAACGCG50450.011.2223755
CTCGCAA75700.011.15063430
TGACGAC82050.010.9689251
TAATTCG28850.010.96111615
AACGCGG54050.010.7324366
ACGCGGA54500.010.6448627
TGCGCGA55700.010.60593214
ACGACCT85100.010.5296273
CTTGACG39450.010.4819691
TCGCACG57100.010.40277530
Produced by FastQC (version 0.11.5)