FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005002270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43882792
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG520170.11853621346608939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA48000.020.6897414
TACGCAC69950.014.9473089
TAGGTCG24000.011.4291753
GCGAACG30400.011.4215613
ACGACCT103400.010.8127063
CTCGCAA89700.010.80132930
TGACGAC97100.010.73294451
GTCTAAC61150.09.9984591
GACGACC123600.09.7203952
TGCGCGA63900.09.67718114
TACCGGT36300.09.47139144
TCGCACG64000.09.2785830
CGGCGAA44200.09.1956081
CGCGAGT67300.09.08516516
GCGATAG36950.08.98542366
ACCGGTA37900.08.98022845
TAACACG72650.08.9393837
TCGCAAA113850.08.90613931
CTACGCA125600.08.8768328
CTCGATT47550.08.76725149
Produced by FastQC (version 0.11.5)