FastQCFastQC Report
Tue 19 Apr 2022
EGAF00005002321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005002321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411499331
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1039400.030.7783991
TCGGAAG1144750.027.8848572
CGGAAGA1393550.022.9056133
TATGCCG704450.09.54995645-49
AGAGCAC3790950.09.0693997
AGCACAC4115550.08.3945689
CGTATGC821450.08.24975645-49
GAAGAGC4198000.08.1917315
CTCGTAT717400.08.1547140-44
GAGCACA4243750.08.1239188
TCTCGTA746550.07.85377840-44
TTACTCG909450.07.31129530-34
TACTCGA949850.07.2782235-39
ATGCCGT947600.07.19587345-49
TGCCGTC939900.07.01726445-49
GCCGTCT976600.06.98830850-54
TCGTATG875850.06.63309140-44
ATCTCGT954950.06.384277340-44
CGTCTGA1120750.06.2792615-19
ACACGTC1146150.06.118432510-14
Produced by FastQC (version 0.11.5)